Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)

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Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae).

Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequencing on the Illumina HiSeq 2000 platfo...

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PREMISE OF THE STUDY Transcriptome-derived simple sequence repeat (SSR) markers were developed in Asparagus cochinchinensis (Asparagaceae). Due to its application in traditional medicine, its wild populations are threatened by over-collection even in protected areas, requiring immediate conservation efforts. METHODS AND RESULTS Based on transcriptome data of A. cochinchinensis, 96 primer pair...

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Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2-13 alleles per locus. The observed and expected heterozygosities were...

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Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)

Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight suga...

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ژورنال

عنوان ژورنال: Genetics and Molecular Research

سال: 2016

ISSN: 1676-5680

DOI: 10.4238/gmr.15038406